Effects of TQ5 on closure time according to PFA-100 analysis and thrombotic platelet plug formation in the mesenteric venules of mice. (A) The shear-induced platelet plug formation in whole blood was determined by CT, as described in the materials and methods section; (B) Mice were administered an intravenous bolus of DMSO (control) or TQ5 (4 mg/kg), and the mesenteric venules were irradiated to induce microthrombus formation (occlusion time), as described in the materials and methods section; Microscopic images (magnification ×400) of DMSO-treated controls (a,b) and the TQ5 (4 mg/kg)-treated groups (c,d) were recorded 5 s (a,c) and 150 s (b,d) after irradiation; (C) Bar diagrams are representative examples of six similar experiments. The arrows indicate platelet plug formation. Data ((A), n = 5 and (C), n = 6) are presented as the means ±S.E.M. * p < 0.05 and ** p < 0.01 compared with the control group.