Skip to main content
. 2017 Apr 27;18(5):920. doi: 10.3390/ijms18050920

Figure 2.

Figure 2

YKL-40 induces IL-18 production and EPC angiogenesis in a time-dependent manner. (A–C) MG-63 cells were treated with YKL-40 (10 ng/mL) for prespecified time intervals, as indicated. IL-18 expression was examined by qPCR, Western blotting, and ELISA immunoassay procedures (n = 4 per group). (D,E) MG-63 cells were treated with YKL-40 (10 ng/mL) for indicated time intervals. CM was collected and applied to EPCs. Capillary-like structure formation and cell migration of EPCs was examined by tube formation and Transwell assay (n = 5 per group). Results are expressed as the mean ± S.E. * p < 0.05 compared with control.