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. 2017 Apr 27;18(5):920. doi: 10.3390/ijms18050920

Figure 4.

Figure 4

The PI3K/Akt signaling pathway regulates YKL-40-induced increases in IL-18 expression and angiogenesis. (A,B) MG-63 cells were pretreated with LY294002 (10 μM) and an Akt inhibitor (10 μM) or transfected with p85 and Akt siRNA for 24 h, then stimulated by YKL-40 for 24 h. IL-18 expression was examined by qPCR and ELISA (n = 4 per group). (C,D) CM was collected and applied to EPCs. Capillary-like structure formation and cell migration of EPCs was examined by tube formation and Transwell assay (n = 5 per group). (E) MG-63 cells were treated with YKL-40 for indicated time intervals, and p85 and Akt phosphorylation were examined by Western blotting. p85 and AKT phosphorylation in each independent experiment was quantified by densitometry (n = 3 per group). (F,G) MG-63 cells were pretreated with a FAK inhibitor or LY294002 for 30 min then stimulated with YKL-40, and p85 and Akt phosphorylation activities were examined by Western blotting (n = 3 per group). Results are expressed as the mean ± S.E. * p < 0.05 compared with control. # p < 0.05 compared with the YKL-40-treated group.