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. 2017 Apr 28;18(5):926. doi: 10.3390/ijms18050926

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison of luteinizing hormone (LH)- and human chorionic gonadotropin (hCG)-induced extracellular-regulated kinase 1/2 (ERK1/2), protein kinase B (AKT) and cAMP-responsive element binding protein (CREB) phosphorylation over 72 h, in human primary granulosa lutein cells (hGLC). (A) Evaluation of pERK1/2, pAKT and pCREB activation by Western blotting. Total ERK served as loading control (images representative of four independent experiments); (B–D) Semi-quantification of pERK1/2, pAKT and pCREB Western blotting signals. § = significantly different to unstimulated (control) at the same time-point; * = significant difference of LH versus hCG; two-way ANOVA and Bonferroni post-test (p < 0.05; means ± standard deviation (SD); n = 4).