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. 2017 Apr 28;18(5):926. doi: 10.3390/ijms18050926

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of LH- and hCG-treated (0–72 h), serum-starved hGLC viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cells maintained in the absence of gonadotropin served as controls. (A) Directly measured absorbance values; (B) Cell viability values expressed as increase or decrease over basal levels. § = significantly different to unstimulated (control) at the same time-point; * = significant difference of LH versus hCG; two-way ANOVA and Bonferroni post-test (p < 0.05; means ± SD; n = 10).