Figure 5.

Comparison of pAKT activation and procaspase 3 cleavage over 72 h, in hGLC maintained under LH- or hCG-treatment in the presence of 17β-estradiol. (A) Evaluation of pAKT and procaspase 3 by Western blotting in cells treated 0–3 days by LH or hCG together with the estrogen. Unstimulated cells were maintained in the presence of 17β-estradiol too. Total ERK and β-actin served as loading controls (images representative of three independent experiments); (B,C) Semi-quantification of pAKT (B) and procaspase 3 (C) Western blotting signals. Data from samples maintained in the presence of 17β-estradiol are represented by bars, while circles were samples in the absence of the estrogens extracted from Figure 1 and Figure 2. ¤ = significantly different to unstimulated (control) at the time-point 0; two-way ANOVA and Bonferroni post-test (p < 0.05; means ± SD; n = 3).