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. 2017 Apr 30;18(5):935. doi: 10.3390/ijms18050935

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Nobiletin inhibited DNA binding activity and gene promoter binding of STAT3. (A) Western blotting analysis of MCF-7 nuclear proteins showing the inhibition of Src/FAK/STAT3 signaling and PXN; (B) EMSA analysis showing inhibition of the DNA binding activity STAT3 to the GAS element; (C) Sequence of the human PXN gene promoter (available online: https://www.ncbi.nlm.nih.gov/nuccore/NG_029820.1?from=5001&to=60333&report=genbank). A GAS element (ttctgggaa) present in the PXN gene (nucleotide sequence 52769-52777) is highlighted; (D) ChIP assay analysis showing the complex formation of STAT3/PXN and inhibition by nobiletin. The relative DNA binding of STAT3 to the PXN gene promoter was expressed as a percentage of the control. Statistical analysis was performed using Student’s t-tests (*** p < 0.001).