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. 2017 Apr 29;18(5):942. doi: 10.3390/ijms18050942

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RE-CE scavenges IR-induced reactive oxygen species (ROS) in HSPCs. Mice were daily treated with the vehicle or RE-CE as described in the Materials and Methods. (A) The total ROS levels in LSKs were presented as the MFI of 2,7-dichlorodihydrofluorescein diacetate (DCFDA); (B) The mitochondria-derived ROS levels in c-kit positive cells were presented as MFI of MitoSox; (C) The levels of superoxide free radicals were presented as the MFI of dihydroethidium (DHE). RE-CE treatment significantly decreased the MFI of DCF, MitoSox, and DHE in irradiated HSPCs. The MFI of DCF, MitoSox and DHE are presented as means ± SEM (n = 5).