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. 2017 Apr 29;18(5):943. doi: 10.3390/ijms18050943

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Hypoxia-induced epithelial-mesenchymal transition (EMT) and expression of stem cell markers in HNSCC. RT-qPCR was performed to analyze mRNA expression levels of EMT (A) and stem cell (B) markers in HNSCC cells following exposure to normoxic and hypoxic conditions for 7 days in the presence or absence of cetuximab (60 nM). The relative amount of analyzed genes is calculated using the 2^−ΔΔCt method and amplification of GAPDH and β-actin were used as an internal standard. Data were normalized to cells cultured in normoxic conditions in each column graph (mean values ± SD; n = 3). * p < 0.05 versus N (normoxia) and ** p < 0.05 versus H (hypoxia) according to Student’s t-test.