Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 29;18(5):943. doi: 10.3390/ijms18050943

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effect of HIF-1α downregulation on EMT profile and expression of stem cell markers in HNSCC. The UT-SCC-14 and LK0412 cells were transiently transfected with either non-targeting siRNA or HIF-1α-specific siRNA and maintained under hypoxia for 72 h. The mRNA expression levels of (A) EMT markers and (B) stem cell markers in HNSCC cells cultured under hypoxia were analyzed by RT-qPCR. The relative amount of analyzed genes is calculated using the 2^–ΔΔCt method and amplification of GAPDH and β-actin were used as an internal control. Data were normalized to cells cultured in hypoxic conditions and transfected with control, non-targeting siRNA in each column graph (mean values ± SEM; n = 3). * p < 0.05 according to Student’s t-test.