Table 1.
Identification of cyanidin-based anthocyanins, their retention times, molecular ions by mass spectrometry (MS) fragmentation, the UV-VISabsorption maxima and the individual anthocyanins content of anthocyanins enriched extract (AEE), calculated as Cy-3-O-samb equivalent (mg/100 g of fresh weight (FW)).
| Peak | HPLC Rt (min) | λmax (nm) | Molecular Ion (MS) m/z+ | Fragment (MS-MS) m/z+ | Identification of Anthocyanins | Anthocyanins Content (mg/100g FW) |
|---|---|---|---|---|---|---|
| 1 | 4.7 | 275 (525) | 743 | 581,449,287 | Cy-3-O-samb-5-gluc | 48.49 ± 0.74 |
| 2 | 6.8 | 275 (516) | 611 | 449,287 | Cy-3,5-digluc | 84.57 ± 1.53 |
| 3 | 10.4 | 271 (518) | 581 | 449,287 | Cy-hexoside-pentoside | 35.34 ± 1.42 |
| 4 | 12.2 | 279 (518) | 581 | 449,287 | Cy-3-O-samb | 255.56 ± 2.23 |
| 5 | 13.0 | 275 (517) | 449 | 287 | Cy-3-O-gluc | 71.18 ± 1.83 |
| Total | 495.16 ± 7.75 |