Figure 2.

Neural differentiation of control and CLN5Y392X iPSC lines. iPS cells were differentiated into neural progenitor cells (NPCs), which grow as neurospheres in a suspension culture (A,B); Neurospheres were further differentiated on poly-l-ornithine/laminin-coated plates (C,D); At day seven, cultures were fixed and stained with the neuron-specific marker microtubule-associated protein 2 (MAP2) (green) (E,F); Bar 400 µm (A,B), 200 µm (C,D) and 50 µm (E,F).