Figure 3.

Subcellular localization of REF and SRPP proteins in Arabidopsis protoplasts using GFP-fluorescence. PEZS plasmids containing REF-GFP or SRPP-GFP fusions were transfected into the protoplasts. As a control a PEZS vector (containing GFP alone) was transfected. REF proteins appear to be gathered in the shadow zone of the cell under the view of Bright Field, and SRPP proteins appear to have diffused GFP fluorescence throughout the cells. GFP was excited with an argon laser at 488 nm and fluorescence images were taken using a confocal laser scanning microscope. Scale bars = 10 μm.