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. 2017 May 4;18(5):977. doi: 10.3390/ijms18050977

Figure 2.

Figure 2

MiR-365 over-expression ameliorated DEX-induced inhibition of osteoblast cell viability and alkaline phosphatase activity. The cells were transfected with miR-365 mimic or miRNA mimic negative control. After 12 h, the cells were treated with 1 µM DEX or vehicle control. (A) CCK-8 assay was performed to test the viability on days 1, 2, and 3 (n = 6). (B) Alkaline phosphatase activity was detected by BCIP/NBT staining on day 7. ** p < 0.01 compared to control; ## p < 0.01 compared to DEX+miR-ctrl. Scale bar: 200 µm.