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. 2017 May 4;18(5):977. doi: 10.3390/ijms18050977

Figure 5.

Figure 5

Histone deacetylase 4 (HDAC4) is a direct target of miR-365 in MC3T3-E1 cells. (A) Schematic diagram of putative miR-365 seeding-site (Response Element, RE) in 3′-UTR of HDAC4 mRNA; (B) pmirGLO-HDAC4 3′-UTR wild-type gene was co-transfected with the negative control or miR-365 mimic into MC3T3-E1 cells respectively. Cells were harvested for quantification of dual luciferase activities at 24 h post transfection (n = 3); (C) qPCR analysis of the changes of the mRNA expression of HDAC4 in MC3T3-E1 cells after treatment with miR-365 mimic or negative control (n = 3); (D) Western blot analysis of the changes in HDAC4 protein in MC3T3-E1 cells after treatment with miR-365 mimic or negative control for 72 h (n = 3). * p < 0.05, ** p < 0.01 compared to control.