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. 2017 May 5;18(5):981. doi: 10.3390/ijms18050981

Figure 2.

Figure 2

Analysis of adipogenic specific proteins. Immunohistochemical analysis of the expression of TMEM 16 (A), ASC-1 (B) and PAT2 (C) was assessed in cells cultured for 21 days in basic medium (BM) as a negative control and in the presence of the Differentiation Medium (DM) or the Differentiation Medium together with melatonin (Melatonin-DM) or with 10−6 vitamin D (Vitamin D 10−6 M-DM) or with 10−8 M vitamin D (Vitamin D 10−8 M-DM), or with both melatonin and vitamin D (Melatonin + Vitamin D 10−6 M-DM, Melatonin + Vitamin D 10−8 M-DM). Nuclei are labelled with 4,6-diamidino-2-phenylindole (DAPI, blue). Scale bars: 40 µm. The figures are representative of five separate experiments. For each differentiation marker, fields with the highest yield of positively stained cells are shown.