Table 1. Sequence validation rates of predictions.

Gene prediction set	Total no. of predictions*	No. of predictions tested	No. sequence validated (%)†
sjc set	197	171	64 (37.4)
Heidelberg set‡	1,266	160	18 (11.3)
Homol-2 set	362	209	28 (13.4)
Homol-0 set§	9,577	204	12 (5.9)
Total predictions	11,402	744	122 (16.4)
Controls	159	159	154 (96.9)

^*See Table 2 for specific numbers from genscan vs. fgenesh predictions

^†Gene predictions were considered validated if the aligned sequence of the PCR product was consistent with a spliced gene model in the region of the prediction

^‡Only 1,266 multiexon predictions from the 2,636 predictions described by Hild et al. (2) were considered for analysis, and, of these, we tested only the 160 with the highest priority scores that did not overlap any genscan or fgenesh predictions tested in the other sets

^§The homol-0 set was selected to be representative of the full range of priority scores