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. 2017 May 11;18(5):1034. doi: 10.3390/ijms18051034

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of MS19 on the expression of IRF5 in LPS-treated RAW264.7 cells. RAW264.7 cells were cultured in medium with LPS (1 µg/mL) or LPS (1 µg/mL) + MS19 (8 µg/mL) for 2 h, and then analyzed for interferon regulatory factor 5 (IRF5) mRNA by qRT-PCR or protein by western blotting. GAPDH mRNA and protein were as normalized standards for IRF5 mRNA and protein in total or cytoplasm proteins, respectively. PCNA protein was as normalized standard for IRF5 protein in nuclear proteins. (A) IRF5 mRNA levels in total cell lysate. Data expressed as mean ± SD; (B) IRF5 protein levels in total proteins of the cells; (C) IRF5 protein levels in cytoplasm proteins of the cells; (D) IRF5 protein levels in nuclear proteins of the cells.