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. 2017 May 12;18(5):1037. doi: 10.3390/ijms18051037

Figure 1.

Figure 1

Isolation of hematopoietic stem and progenitor cells (HSPC) from mouse bone marrow. The gating strategies used to identify hematopoietic stem cells (HSC) and various progenitors are shown. The areas delineated by the black boxes in the scatter plots and solid black lines in the histograms indicate the population of cells gated. Dashed arrows point to further gating of these cells. Shaded areas in the histograms depict the isotype control staining. Lineage markers (Lin) included CD3ε, B220, CD11b, Ly-6G and TER-119. HSPC, hematopoietic stem and progenitor cells; LT-HSC, long-term reconstituting hematopoietic stem cell; ST-HSC, short-term reconstituting hematopoietic stem cell; HSC, hematopoietic stem cell; MPP, multipotent progenitor; HPC, hematopoietic progenitor cell; lymphoid-primed multipotent progenitors (LMPP), lymphoid-primed multipotent progenitor; common lymphoid progenitors (CLP), common lymphoid progenitor; common myeloid progenitors (CMP), common myeloid progenitor; GMP, granulocyte-macrophage progenitor; MEP, megakaryocyte-erythrocyte progenitor; LS−K, Lineage marker− Sca1− c-Kit+; LSK, Lineage marker− Sca1+ c-Kit+; Flt3, fms-like tyrosine kinase 3; IL-7R, interleukin-7 receptor. SSC, side light scatter; FSC, forward light scatter.