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Complementation of the HXK-deficient yeast triple mutant YSH7.4-3C (hxk1, hxk2, glk1) with MeHXK2. −URA + Glc: the medium contained d-glucose as the sole carbon source and lacked uracil; −URA + Fru: the medium contained d-fructose as the sole carbon source and lacked uracil; MeHXK2: the pDR195-MeHXK2 vector was transformed into YSH7.4-3C; PRD195: the empty pDR195 vector was transformed into YSH7.4-3C; YSH7.4-3C: the mutant yeast cells without any vector transformation.
