Figure 4.

Circulating MPs are represented on a forward scatter/side scatter dot plot. (A) Total MPs are defined as events with a size of 0.1 to 1.0 µm gated in the window using 1-µm diameter calibrant beads as the interior criterion prior to the sample testing; (B) Size-selected events are plotted according to their fluorescence for specific HepPar1 binding on a fluorescence plot. Events included in the K4 section were considered HepPar1+ MPs. J is the gate of definition of fluorescent megamix beads.