Figure 3.

Relative expression levels of potential reference genes determined for the same amount of isolated mRNA. The relative expression levels of eight potential reference genes were determined by RT-qPCR in samples obtained from the same amount of reverse transcribed isolated messenger RNA from skeletal muscle of 2-, 4- and 9-week-old (2, 4, 9 w) normal mice. Data are the mean ± SD of the indicated n number of samples per group, each one representing a different animal. Normal distribution was evaluated by the D’Agostino-Pearson test and ACTB, HPRT1, PPIA and YWHAZ data were log-transformed to achieve normality. Different letters denote significant differences by age determined by one-way ANOVA followed by the Bonferroni post-test. A p-value lower than 0.05 was considered statistically significant. ns stands for non-significant.