Table 1.
Amplification efficiencies and R2 values of standard curves of potential reference genes and target genes for reverse transcription-quantitative PCR (RT-qPCR) experiments in skeletal muscle of growing mice. Standard curves were constructed from six cDNA dilutions of a pool of samples of 2-, 4- and 9-week-old normal animals. Amplification efficiencies of each pair of primers were calculated from the slope of the curves, obtained by linear regression, according to the equation E% = (10(−1/slope) − 1) × 100%.
| Gene Symbol | Efficiency (%) | R2 |
|---|---|---|
| ACTB | 101 | 0.982 |
| B2M | 98 | 0.998 |
| GAPDH | 95 | 0.999 |
| GHR | 93 | 0.995 |
| GSK3B | 91 | 0.995 |
| HPRT1 | 105 | 0.991 |
| IGF1 | 108 | 0.996 |
| PPIA | 101 | 0.998 |
| RN18S | 101 | 0.998 |
| RPL13A | 100 | 0.998 |
| YWHAZ | 99 | 0.980 |