Table 4.
Sequences of RT-qPCR primers designed for the detection of potential reference genes and target genes in mice.
| Gene Symbol | Gene Name | GenBank Accession Number | Primer Sequence (5′–3′) a |
|---|---|---|---|
| ACTB | Actin, β | NM_007393.5 | F: GTGCCCATCTACGAGGGCTATGCT |
| R: TACCCAAGAAGGAAGGCTGGAAAA | |||
| B2M | β-2 microglobulin | NM_009735.3 | F: AAGTATACTCACGCCACCCA |
| R: AAGACCAGTCCTTGCTGAAG | |||
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | NM_008084.3 | F: AGTGCCAGCCTCGTCCCGTAG |
| R: GTGCCGTTGAATTTGCCGTGAGTG | |||
| GHR | Growth hormone receptor | NM_001286370.1 | F: CCAACTCGCCTCTACACCG |
| R: GGGAAAGGACTACACCACCTG | |||
| GSK3B | Glycogen synthase kinase 3β | NM_019827.6 | F: CCACCATCCTTATCCCTCCAC |
| R: GTATCTGAGGCTGCTGTGGC | |||
| HPRT1 | Hypoxanthine guanine phosphoribosyl transferase | NM_013556.2 | F: CAGTCCCAGCGTCGTGATTA |
| R: TCGAGCAAGTCTTTCAGTCCT | |||
| IGF1 | Insulin-like growth factor 1 | NM_010512.4 | F: CCAAACACAATTCTCCTTCC |
| R: GCTACAGCAACCTGTGATTG | |||
| PPIA | Peptidylprolyl isomerase A | NM_008907.1 | F: GCGTCTCCTTGAGCTGTT |
| R: AAGTCACCACCCTGGCAC | |||
| RN18S | 18S ribosomal RNA | NR_003278.3 | F: ACGGACAGGATTGACAGATT |
| R: GCCAGAGTCTCGTTCGTTAT | |||
| RPL13A | Ribosomal protein L13A | NM_009438.5 | F: TGACAAGAAAAAGCGGATGGTG |
| R: GCTGTCACTGCCTGGTACTT | |||
| YWHAZ | Tyr 3-monooxygenase/Trp 5-monooxygenase activation protein, Z polypeptide | NM_011740.3 | F: CCAGGACCTAAAAGGGTCGG |
| R: ACACACCGAACTGTTGTCGT |
a F = forward primer, R = reverse primer.