Skip to main content
. 2017 May 19;18(5):1063. doi: 10.3390/ijms18051063

Figure 7.

Figure 7

The effect of Rb2 on OA-induced hepatic autophagy dysfunction through the upregulation of sirt1 expression and AMPK phosphorylation. HepG2 cells (A) and primary mouse hepatocytes (B) were pretreated with 50 µmol/L Rb2 for 4 h before OA (33.3 mmol/L glucose in combination with 1 mmol/L OA for HepG2 and 2 mmol/L OA for primary hepatocytes) exposure for 12 h, and levels of LC3-II, P62, sirt1, p-AMPK, and AMPK were detected by Western blotting analysis. Data are expressed as mean ± SE from three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with vehicle-treated group (Control). # p < 0.05 and ## p < 0.01 compared with OA-treated group (Control).