Figure 5.

Nrf2 influences OS mediated gene expression in PDLSC. The PDLSCs were treated with 1000 µmol/L H₂O₂ for 2 h. The PDLSCs were transfected with lentiviral Nrf2 for overexpression (1000 + ACT: overexpression of Nrf2 at 1000 µmol/L H₂O₂ for 2 h) or a lentiviral control (1000 + ACT-NC: overexpression negative control at 1000 µmol/L H₂O₂ for 2 h), and they were silenced with Nrf2 shRNA (1000 + shRNA: silence of Nrf2 at 1000 µmol/L H₂O₂ for 2 h) or control shRNA (1000 + shRNA-NC: silence negative control at 1000 µmol/L H₂O₂ for 2 h). (A) Relative gene expression levels of Nrf2 and its related downstream molecules, HO-1, NQO1 and γ-GCS, as determined by real-time PCR analysis; (B) Western blot for the anti-oxidative molecules, Nrf2, HO-1, NQO1 and γ-GCS and (C) their quantification. Data are presented as the means ± SD, n = 5. * p < 0.05 and ** p < 0.01 represent significant differences between the indicated columns.