Table 1.
Expression message for neurotrophic factors and p75 in pericytes and other cell types. Publicly available microarray expression data available at the Gene Expression Omnibus (GEO) were analyzed. Expression data from non-cultured pericytes (ncPCs; accession GSE71535) were compared with data from cultured pericytes (cPCs) expanded in pericyte medium (PC medium) or mesenchymal stromal cell (MSC) medium (accessions GSM1655126, GSM1655127, GSM1655128, GSM1655129, GSM1655130, and GSM1655131), adipose tissue-derived MSCs (ATMSCs (Adipose Tissue-Derived MSCs); accessions GSM1655122, GSM1655123, and GSM1655124), human umbilical vein endothelial cells (HUVECs (Human Umbilical Vein Endothelial Cells); accessions GSM418611, GSM418615, and GSM418619), and peripheral blood white blood cells (PBWBCs (Peripheral Blood White Blood Cells); GSM469524, GSM469528, GSM469532, GSM469536, and GSM469540). Numbers in the table’s cells represent fluorescence intensity in the microarray spot corresponding to the specified probe after quantile normalization and log2 transformation, which is proportional to expression of the specified transcripts. Cells with expression values were colored according to expression intensity as shown in the scale bar. Note that three different probes (and, consequently, spots) for BDNF, and three different probes for GDNF were present in the microarrays analyzed. In the case of GDNF, two of the probes identify its transcript variant 1, and one of the probes identifies a transcript for a precursor form of GDNF.
| Probe Name | Gene Symbol | Description | ncPCs | cPCs (PC Medium) | cPCs (MSC Medium) | ATMSCs | HUVECs | PBWBCs |
|---|---|---|---|---|---|---|---|---|
| A_23_P115190 | NGF | nerve growth factor (β polypeptide) | 7.721 | 4.458 | 6.394 | 7.998 | 3.967 | 4.790 |
| A_23_P127891 | BDNF | brain-derived neurotrophic factor, transcript variant 1 | 6.635 | 7.903 | 11.047 | 8.457 | 6.650 | 2.812 |
| A_32_P7316 | BDNF | brain-derived neurotrophic factor, transcript variant 1 | 2.739 | 2.975 | 8.517 | 5.321 | 4.026 | 2.572 |
| A_23_P127891 | BDNF | brain-derived neurotrophic factor, transcript variant 1 | 6.635 | 7.903 | 11.047 | 8.457 | 6.650 | 2.812 |
| A_23_P360797 | NTF3 | neurotrophin 3, transcript variant 2 | 8.034 | 3.479 | 3.916 | 8.211 | 4.513 | 3.945 |
| A_23_P4899 | NTF4 | neurotrophin 4 (NTF4) | 2.052 | 2.046 | 5.979 | 2.293 | 2.266 | 2.352 |
| A_24_P25544 | GDNF | glial cell derived neurotrophic factor, transcript variant 1 | 2.277 | 2.294 | 4.050 | 2.979 | 1.758 | 1.948 |
| A_23_P167683 | GDNF | glial cell derived neurotrophic factor, transcript variant 1 | 4.375 | 3.359 | 4.955 | 2.883 | 1.868 | 1.852 |
| A_32_P377880 | GDNF | glial cell derived neurotrophic factor, precursor | 7.660 | 7.685 | 9.894 | 6.382 | 1.790 | 2.378 |
| A_23_P90359 | NRTN | Neurturin | 2.839 | 2.461 | 4.375 | 2.853 | 2.636 | 3.578 |
| A_23_P410507 | PSPN | Persephin | 8.082 | 7.490 | 6.954 | 6.349 | 6.620 | 8.475 |
| A_23_P389897 | NGFR | nerve growth factor receptor (p75) | 10.493 | 2.299 | 3.673 | 5.042 | 2.448 | 3.644 |
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