Skip to main content
. 2017 May 18;18(5):1088. doi: 10.3390/ijms18051088

Figure 4.

Figure 4

The ERK and p38 MAPK pathways are involved in the regulation of salinomycin-induced autophagy in prostate cancer cells. (A,B) ERK and p38 MAPK protein expression. (A) PC-3 cells. (B) LNCaP cells. Cells were treated with 2 µM salinomycin for the indicated times. After treatment, total cell lysates were subjected to SDS-PAGE for Western blot analysis; (C) the effect of PD98059 on salinomycin-induced autophagy; (D) the effect of SB203580 on salinomycin-induced autophagy. Cells were pretreated with 10 µM PD98059 or 10 µM SB203580 for 1 h, and acridine orange (AO) stained cells were evaluated by flow cytometry. All data were representative of at least three independent experiments. Data were shown as the mean ± SD. * p < 0.05.