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. 2017 May 19;18(5):1092. doi: 10.3390/ijms18051092

Figure 9.

Figure 9

Suberoylanilide hydroxamic acid (SAHA) and combinatorial SFN + WA promote changes in apoptotic genes at the mRNA level. (A) qRT-PCR was used to determine the mRNA expression of HDAC1 in MDA-MB-231 triple-negative breast cancer cells in comparison to the 3.0 µM optimal concentration of SAHA. SFN and WA concentrations are 5.0 and 1.0 µM respectively; (B) mRNA expression of the pro-apoptotic BAX is shown in MDA-MB-231 cells; (C) qRT-PCR shows the expression of the anti-apoptotic BCL-2 in MDA-MB-231 cells; (D) HDAC1 mRNA expression in MCF-7 cells shows changes with the incorporation of the chosen compounds; (E) mRNA expression of the pro-apoptotic gene BAX is upregulated by combinatorial SFN and WA; (F) The anti-apoptotic gene BCL-2 shows a downward progression in MCF-7 cells with the incorporation of the indicated drugs and compounds (n = 3: SEM, * p < 0.05, ** p < 0.01, *** p < 0.001). SEM: standard error of mean.