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. Author manuscript; available in PMC: 2017 Jun 2.
Published in final edited form as: Sci Signal. 2016 Mar 1;9(417):ra23. doi: 10.1126/scisignal.aaa9227

Fig. 1. YAP localizes to the central spindle and to the midbody ring.

Fig. 1

(A and B) Endogenous YAP (A) and LATS1 (B) localize to the central spindle of MCF-10A cells in anaphase. YAP (Cell Signaling Technology) and LATS1 (Bethyl Laboratories Inc.), green; tubulin, red; and 4′,6-diamidino-2-phenylindole (DAPI), blue. (C to F) YAP (Cell Signaling Technology) (C) and LATS1 (Bethyl Laboratories Inc.) (D) localize to the midbody ring of MCF-10A cells during cytokinesis (green); inset shows midbody ring. Other YAP antibodies [Abcam, (E); Sigma-Aldrich, (F)] show localization to the mid-body ring in MCF-10A cells (green). (G) YAP localizes to the midbody ring in HeLa cells (green). (H) YAP Ser127 (Cell Signaling Technology) localization in MCF-10A. (I to M) Overexpressed exogenous EGFP-YAP (I) or Flag-YAP (J) in HeLa cells localizes to the midbody ring. YAP (red) colocalizes with Cep55 (K), MST (L), and LATS1 (green) (M) at the midbody. Arrows indicate the central spindle or midbody region. Scale bars, 5 μm. (A) to (J) are representative of 95 images obtained from three independent experiments. (K) to (M) are representative of 35 images obtained from three independent experiments.