Table 2.
Tau Antibodies Used in This Study
| Antibody | Isotype | EC50, nmol/L |
Epitope | Source | |
|---|---|---|---|---|---|
| Recombinant tau (ELISA) | PHF tau (ELISA) | ||||
| 6C5 | Human IgG1/λ | 0.03 | 0.04 | 125–131 | Biogen (Cambridge, MA) |
| 40E8 | Human IgG1/λ | >500 | 0.2 | 195–212 (pS202 and pT205) | Biogen |
| 4E4 | Human IgG1/λ | 13 | >100 | 385–395 | Biogen |
| Tau13 | Mouse IgG1 | 0.02 | 0.01 | 2–18 | Santa Cruz Biotechnology (Dallas, TX) |
| HT7 | Mouse IgG1/κ | 0.1 | 0.06 | 159–163 | Thermo Fisher Scientific (Waltham, MA) |
| Tau46 | Mouse IgG1 | 0.06 | 0.03 | 404–441 | Santa Cruz Biotechnology |
| pS396 | Rabbit polyclonal | >500 | 1.8 | pS396 | Life Technologies (Carlsbad, CA) |
EC50, 50% effective concentration; ELISA, enzyme-linked immunosorbent assay; PHF, paired helical filament.