Figure 5.

Decreased proliferation in Bmal1^fx/fx;Tek-Cre mice. Bone marrow mononuclear cells were isolated from WT and Bmal-1^fx/fx;Tek-Cre mice and were propagated in methylcellulose semisolid cultures; the number of colonies was enumerated after one week. Representative images and bar charts showing decreases in CFU-GM (granulocyte monocyte) (A), CFU-GEMM (granulocyte, erythrocyte, monocyte/macrophage, megakaryocyte) (C), and CFU-G (granulocyte) (D), and an increase in CFU-monocytes (M) in Bmal-1^fx/fx (B). Data are expressed as means ± SEM. n = 5 WT; n = 6 Bmal1^fx/fx;Tek-Cre. ^∗P < 0.05 versus WT. Scale bars = 200 μm.