Figure 6.

Bmal1^fx/fx;Tek-Cre mice show an increase in nitrative stress. The retinas of WT and Bmal1^fx/fx;Tek-Cre mice after an ischemia/reperfusion injury were paraffin-embedded, sectioned, and stained for nitrotyrosine. A: Representative images of retinal mounts showing a profound increase in nitrotyrosine staining in injured Bmal1^fx/fx;Tek-Cre mice (arrows). B: Quantification fluorescence intensity for nitrotyrosine. Data are expressed as means ± SEM. n = 5 WT; n = 6 Bmal1^fx/fx;Tek-Cre. ^∗P < 0.05. Scale bars = 20 μm. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; PR, photoreceptor layer.