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. 2017 Jun;187(6):1258–1272. doi: 10.1016/j.ajpath.2017.02.009

Figure 2.

Figure 2

The effect of pyrophosphate (PPi) and bisphosphonates on the acute dystrophic cardiac calcification (DCC) phenotype of Abcc6−/− mice. A: Various amounts of PPi were i.p. injected in Abcc6−/− mice daily for 7 days after inducing DCC. A single injection (inj.) of 28 μmol/kg (12.5 mg/kg) of PPi immediately after cryoinjury was sufficient to inhibit DCC. The level of calcification was measured as total ventricular calcium. B: Two bisphosphonates, alendronate (Aln) and etidronate (Etd), were tested against DCC in Abcc6−/− mice. Etidronate (8 μmol/kg per day or 2.0 mg/kg per day) significantly inhibits cardiac calcification, whereas alendronate (0.062 μmol/kg per day or 0.02 mg/kg per day) has no significant effect as compared to saline injections. The number of mice per group is shown. Data are expressed as means ± SEM (A and B). ∗∗P < 0.01, ∗∗∗P < 0.001 versus saline.