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. 2017 Jun;187(6):1258–1272. doi: 10.1016/j.ajpath.2017.02.009

Figure 4.

Figure 4

Histological evaluation of vibrissae calcification of Abcc6−/− mice after pyrophosphate (PPi) supplementation. The effect of daily PPi or saline injections on the calcification of vibrissae (chronic pseudoxanthoma elasticum–like phenotype) of Abcc6−/− mice was evaluated by Alizarin S Red staining (red to dark red color). A: Representative images of whiskers with approximately 15 vibrissae are shown for the preonset and postonset treatment groups with saline controls. A higher magnification image of a selected vibrissa (arrows) is shown. Arrowheads point to discrete calcification. B: The relative area occupied by calcification, as visualized by Alizarin red staining, was also quantified by morphometric analysis using the ImageJ software and show significant reduction between the saline and PPi treatments. C: For comparison purposes, representative images of whiskers from untreated Abcc6−/− mice are included. A higher magnification image of a selected vibrissa (arrows) is shown. The number of mice per group is shown. Data are expressed as means ± SEM. ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 versus saline. Scale bars = 500 μm (A and C). Original magnification, ×200 (higher magnification images).