Figure 7.

Histological evaluation of vibrissae calcification in Abcc6^−/− transgenic mice. The positive effects of both transgenes (normal ABCC6 and R1314W variant) on the calcification of vibrissae (chronic pseudoxanthoma elasticum–like phenotype) was evaluated by Alizarin Red S staining (red to dark red color). A: Representative images of whiskers from 3- to 18-month-old Abcc6^−/− transgenic (Tg) mice carrying the normal human ABCC6 gene are shown. B: Representative images are from whiskers of Abcc6^−/− mice with the ABCC6 R1314W transgene are shown. A higher magnification image of a selected vibrissa (arrows) is shown below each larger panel (A and B). Arrows within the higher magnification images point to discrete calcification spots (A and B). Scale bars = 500 μm (A and B). Original magnification, ×200 (higher magnification images).