Fig. 2.

METH causes increases in FasL expression in GABAergic ENK-containing neurons. Double-labeling immunohistochemistry was performed as in Materials and Methods (see Supporting Text). Antibodies against NeuN, GAD, ENK, and SP were selected to costain with the FasL antibody 24 h after METH injection. METH caused colocalization of NeuN and FasL (Upper Left), GAD and FasL (Upper Right), and ENK and FasL (Lower Left) (yellow cells under Overlap). However, METH did not cause any SP-positive cells to express FasL (Lower Right). (Bar = 30 μm.)