Figure 3.

The MAR epitope is encoded by human TRPM1 exons 9 and 10. HEK cells, transfected with a series of EGFP–human TRPM1 constructs, were tested for immunofluorescence with both MAR sera. (A) Top row: EGFP immunofluorescence showing transfected cells. Second row: MAR serum 1 immunofluorescence. Third row: superimposition of EGFP (green) and MAR serum (red) immunofluorescence, with colocalization appearing yellow. Bottom row: differential interference contrast (DIC) image of the coverslip, showing TRPM1-transfected, and untransfected control cells. (B) Immunofluorescence analysis of HEK transfected cells using the same constructs as in (A), but with MAR serum 2. Serum 2 reacts with the K361-P435 fragment, whereas serum 1 does not. Scale bars: 10 μm.