Figure 2.

(a) A schematic of the sample under biaxial stretch; (b) Stained aortic media. Dark regions in this tissue section denote elastic fibers (Layer I), and light colored regions denote interlamellar zones, including collagen bundles, fine elastic fibers and smooth muscle cells (SMCs) (Layer II); (c) Volume fraction computation steps, including radial strip extraction, conversion to black and white and, finally, counting the number of pixels of the dark (Layer I) and light (Layer II) areas, as well as finding their proportion regarding the total number of pixels.