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ERα and ERβ support ERE- and AP-1 site-dependent transcription with synthetic and natural promoters. Templates containing two EREs (Top) or two AP-1 sites (Middle) upstream of the adenovirus E4 promoter, or a fragment of the collagenase-1 promoter with the consensus AP-1 site at -73/-60 (Bottom), were assembled into chromatin and transcribed in the presence of ERα or ERβ and ligands, as indicated. E2, 17β-estradiol; OHT, 4-hydroxytamoxifen; ICI, ICI164384; Ral, raloxifene.
