Figure 6.

Gas6 modulated the Th2-inducing properties of isolated dendritic cells via Axl. Bone marrow–derived DCs generated from naive WT, Axl−/−, and Mertk−/− mice were treated with Aspergillus antigen and Gas6 and co-cultured with naive CD4 T cells. After co-culture, CD4 cells were activated with anti-CD3 and anti-CD28 before cytokine analysis. Transcript expression of IFN-γ, IL-4, and IL-13 was determined using quantitative PCR. Results are expressed as the mean ± SEM for n = 3 independent experiments in which a minimum of three mice per group were used to isolate DCs and T cells. *P < 0.05 and **P < 0.01.