Table 1. Modification of the RalF C terminus disrupts ARF1-GFP localization to the Legionella-containing vacuole.

		ARF1-GFP localization,* % of vacuoles positive
Strain	Plasmids	Average	SD
Lp01	None	23.8	6.3
Lp01 ΔralF	Vector	1.3	1.2
Lp01 ΔralF	pralF	42.1	6.4
Lp01 ΔralF	pralF-M45	1.3	1.1
Lp01 ΔralF	pralF1-339	0.0	0.0
Lp01 ΔralF ΔdotA	pralF	0.0	0.0

^*Mouse bone marrow-derived macrophages producing ARF1-GFP were infected with the indicated Legionella strains for 1 h, and GFP-ARF1 localization was assayed by fluorescence microscopy. Recruitment of ARF1-GFP to the Legionella-containing vacuoles was scored, and values are presented as the percent of vacuoles that stained positive for ARF1-GFP. Indicated are the average and SD for an experiment performed in triplicate in which a minimum of 50 vacuoles were scored for each sample.