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. Author manuscript; available in PMC: 2018 Mar 1.

Published in final edited form as: Angew Chem Int Ed Engl. 2017 Jan 27;56(10):2763–2766. doi: 10.1002/anie.201612079

Table 2.

Michaelis–Menten parameters for carbohydrate cleavage.

Peptide sequence	pNP-α-L-fucoside^[a]			pNP-β-D-glucoside^[a]

	K_M (mM)	k_cat (min⁻¹)	k_cat/K_M (M⁻¹min⁻¹)	K_M (mM)	k_cat(min⁻¹)	k_cat/K_M (M⁻¹min⁻¹)
CuGGH-tOL-NH₂	0.36 ± 0.08	1.20 ± 0.17	3360 ± 310	2.25 ± 0.62	0.51 ± 0.08	245 ± 34
CuGGH-tOL-OH	0.51 ± 0.07	0.84 ± 0.03	1680 ± 240	2.94 ± 0.31	0.93 ± 0.28	376 ± 25
CuGGH-OL-NH₂	0.35 ± 0.02	0.56 ± 0.08	1630 ± 310	2.16 ± 0.08	1.07 ± 0.13	514 ± 37
CuGGH-OL-OH	0.42 ± 0.06	0.44 ± 0.05	1380 ± 300	2.12 ± 0.17	0.32 ± 0.08	150 ± 31
CuGGH	1.17 ± 0.13	1.49 ± 0.12	1270 ± 36	0.72 ± 0.18	1.40 ± 0.34	1930 ± 120

^[a]

Reactions were performed with 5 μM metallopeptides, 1 mM ascorbate and 1 mM H₂O₂ in 50 mM sodium phosphate buffer (pH 7.4) at 37°C.