Fig. 3.

Truncated AP-Cav peptides block recombinant eNOS activity and eNOS binding to Cav-1. (A) Purified eNOS was preincubated with either l-nitroarginine methyl ester (1 mM) or the peptides (Cav, Cav-AB, or Cav-A; 1-25 μM) for 15 min, and NOS activity was assessed as described in Methods. Data represent duplicate determinations from a single experiment that was repeated twice. (B) Confluent BAEC were treated for 6 h with AP, AP-Cav, AP-Cav-AB, AP-Cav-A, or AP-Cav-B (10 μM). Cells were lysed, Cav-1 was immunoprecipitated, and eNOS coassociation was detected by Western blot analysis. Experiments were performed in duplicate, and typical data are shown. IP, immunoprecipitation; WB, Western blot.