Figure 2.

(a) Color formation of immobilized alkoxysilane-derivatized Fast Blue RR upon incubation with MPO and wound fluids: alkoxysilane-derivatized Fast Blue RR was polymerized and the resulting enzyme-responsive siloxane layer incubated with different enzyme activities of pure MPO, infected and non-infected wound fluids as well as different concentrations of hemoglobin over a time period of 30 min and 60 min. The blue color formation upon conversion of the substrate was quantified with a ColorLite sph850 spectrophotometer and is given as in delta E values. Two-sample t-tests assuming equal variances were performed, p-values equal or less than 0.001 were considered as significant (***). (b) The hemoglobin concentration of the infected and non-infected wound fluid was determined prior to the immobilization experiments.