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Detection and quantitation of covalent intermediates in the reactions of AHAS II mutant Trp464Leu. Steady-state intermediate distribution of AHAS II mutant Trp464Leu in the presence of 100 mM pyruvate (A) or 50 mM pyruvate and 50 mM 2-ketobutyrate (B) in 0.1 M KPi at 37°C. Reactions were quenched and analyzed as in Fig. 3, except that the fingerprint region is zoomed 5- or 8-fold.
