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. 2005 Jan 10;102(3):797–801. doi: 10.1073/pnas.0409065102

Fig. 4.

Fig. 4.

Identification of immunogens and mAbs that circumvent Ab-dependent enhancement of entry and biochemical analysis of the mechanism of enhancement. (a) Neutralization profile of purified IgG from mice vaccinated with a DNA expression vector encoding a secreted form of S(Urbani) terminated at amino acid 1153, S(1153), compared against the indicated human or civet pseudoviruses. (b) (Right) Biochemical interaction of purified IgG from S(1153) DNA-immunized mice with native, expressed S(Urbani) and S(SZ3) proteins by immunoprecipitation (IP) with S(1153) IgG. (Left) The total amount of S protein in the same volume of cell lysates was assessed by Western blotting (Input). Mice were immunized with a S(Urbani) expression vector terminated at amino acid 1153. (c) mAb S110 inhibits S(Urbani) and does not enhance civet pseudovirus entry. S110 or an isotype control Ab (control) was incubated with the indicated pseudoviruses, and inhibition was assessed as described for Fig. 1. The species origin of the S for pseudovirus is shown above each panel (human or civet) in a and c.