Table 1. Candidate housekeeping genes and their primer sequences for qRT-PCR.

Accession	Gene name	Gene symbol	Biological function	Primer sequence (5′–3′)	Tm (°C)	Product size (bp)	Efficiency (%)
c85865_g1	Elongation factor-1α	EF-1α	Essential component of the eukaryotic translational apparatus	f: CGAGATCAAGGAGAAGTGCG	58.5	182	101.4
				r: CAACGGTCTGCTTCATGTCA
c154556_g1	α-actin	ACT	Cytoskeletal structural protein	f: CGAGAACAGGTACACGCAAT	58.5	166	97.5
				r: GTTGAAGGACATGCGGAACT
c104226_g1	Cytochrome c oxidase subunit 1	COX1	Respiratory electron transport chain of mitochondria	f: CTCCTGATATAGCTTTCCCTCG	58.5	165	97.7
				r: CTACAGAACCACCAGCATGAG
c205682_g1	Nicotinamide adenine dinucleotide dehydrogenase [ubiquinone] 1α subcomplex subunit 7	NDUFA7	Electron transport in the respiratory chain	f: GGGAGACGGGAAAACTTGAC	58.5	152	99.8
				r: AGGGAGGTGACAATTAGCCA
c121877_g2	60S ribosomal protein L5	RL5	60S ribosomal subunit	f: GTGGAGGATGAGGATGGACA	58.5	184	96.0
				r: CTGCCTTGTACTCGTTGGTC
c64332_g1	60S ribosomal protein L28	RL28	60S ribosomal subunit	f: CGTGCGTAACATCACCAAGA	57.5	151	102.4
				r: CACCACAGCTACCACACATT
c149072_g1	Glyceraldehyde 3-phosphate dehydrogenase	GAPDH	Glycolytic enzyme	f: CTCTACCAGTCAACGCTCCA	58.5	138	97.6
				r: AATGCGACACCCATCAGAGA
c150134_g1	β-tubulin	TUBB	Structural protein	f: CACTTTCGTGGGCAACTC	57.5	170	96.0
				r: ACTCGGACACCAGGTCGT
c64251_g1	40S ribosomal protein S25	RS25	40S ribosomal subunit	f: ACAAGATGCTGAAGGAGGTG	57.5	224	99.5
				r: ACGCCAGACAAACATGAAAA
c112836_g1	40S ribosomal protein S8	RS8	40S ribosomal subunit	f: TGGTGAAGTCCTGCATCG	57.5	111	97.6
				r: CCTGGGCTGACAGTTTGA
c68556_g1	Ubiquitin-conjugating enzyme E2	UBE2	Protein degradation	f: TTCCTGGACAACTGTCATTCT	57.5	378	100.3
				r: CCTTTCCTCCCTACATTATCTT
c105544_g1	Histone H3	HH3	Essential structure of nucleosome	f: TACAGGCAGCAGATCAGGTT	56.5	366	99.0
				r: CCAGGAAAGTAAGGAGCAGAG
c45955_g1	Peptidyl prolyl cis-trans isomerase A	PPIA	Immunoregulation	f: ATCAGAACACGCTTTCCTTT	57.5	235	97.8
				r: CCAAACCCTTTCTCACCAG