Table 2. Tissue-specific expression profiles of candidate reference genes.
Data are shown as raw Cq values and represented as mean ± standard error (SE).
| Gene | All tissues | Gill | Hemocyte | Intestine | Leiblein | Liver | Kidney | Mantle | Gonad |
|---|---|---|---|---|---|---|---|---|---|
| EF-1α | 20.70 ± 0.38 | 19.14 ± 0.36 | 21.81 ± 0.74 | 23.00 ± 1.35 | 22.48 ± 0.92 | 20.35 ± 0.46 | 20.73 ± 0.68 | 16.98 ± 0.61 | 21.09 ± 0.46 |
| ACT | 26.50 ± 0.47 | 25.20 ± 0.45 | 28.51 ± 0.77 | 29.24 ± 1.35 | 28.24 ± 0.85 | 26.88 ± 0.52 | 26.56 ± 0.73 | 20.57 ± 0.44 | 26.80 ± 0.55 |
| COX1 | 19.26 ± 0.41 | 17.36 ± 0.31 | 20.39 ± 0.97 | 21.47 ± 1.25 | 21.49 ± 0.91 | 18.57 ± 0.56 | 19.10 ± 0.77 | 15.36 ± 0.48 | 20.32 ± 0.68 |
| NDUFA7 | 26.92 ± 0.39 | 24.69 ± 0.32 | 28.42 ± 0.79 | 29.49 ± 1.19 | 28.48 ± 1.00 | 26.54 ± 0.56 | 27.40 ± 0.60 | 23.41 ± 0.73 | 26.94 ± 0.47 |
| RL5 | 23.14 ± 0.41 | 21.92 ± 0.42 | 24.19 ± 0.81 | 25.91 ± 1.45 | 24.57 ± 0.95 | 22.83 ± 0.44 | 23.89 ± 0.67 | 18.74 ± 0.58 | 23.05 ± 0.53 |
| RL28 | 22.59 ± 0.37 | 21.47 ± 0.41 | 23.65 ± 0.52 | 24.96 ± 1.19 | 24.18 ± 0.87 | 22.13 ± 0.42 | 23.42 ± 0.75 | 18.63 ± 0.58 | 22.31 ± 0.61 |
| GAPDH | 24.14 ± 0.42 | 21.60 ± 0.44 | 24.18 ± 0.71 | 26.95 ± 1.11 | 24.98 ± 0.95 | 24.19 ± 0.54 | 24.72 ± 0.59 | 20.11 ± 0.63 | 26.44 ± 0.84 |
| TUBB | 25.95 ± 0.43 | 23.16 ± 0.58 | 26.72 ± 0.35 | 25.78 ± 1.37 | 24.95 ± 0.72 | 23.42 ± 0.78 | 26.08 ± 0.80 | 28.00 ± 0.71 | 29.48 ± 1.33 |
| PPIA | 25.56 ± 0.41 | 24.43 ± 0.35 | 26.15 ± 0.57 | 28.41 ± 1.19 | 26.74 ± 1.03 | 24.78 ± 0.72 | 25.32 ± 0.56 | 21.67 ± 0.71 | 26.99 ± 1.20 |
| RS25 | 24.95 ± 0.39 | 24.27 ± 0.26 | 24.95 ± 0.42 | 27.18 ± 1.39 | 26.22 ± 0.91 | 24.42 ± 0.62 | 25.70 ± 0.80 | 20.59 ± 0.45 | 26.30 ± 0.82 |
| UBE2 | 26.75 ± 0.48 | 24.49 ± 0.46 | 26.60 ± 0.79 | 29.71 ± 1.46 | 28.58 ± 0.85 | 26.65 ± 0.57 | 27.19 ± 1.11 | 22.09 ± 0.88 | 28.67 ± 1.18 |
| RS8 | 24.15 ± 0.43 | 22.95 ± 0.61 | 24.45 ± 0.77 | 26.74 ± 1.52 | 25.80 ± 0.79 | 23.50 ± 0.57 | 24.57 ± 0.85 | 19.81 ± 0.55 | 25.42 ± 0.97 |
| HH3 | 30.36 ± 0.41 | 28.45 ± 0.42 | 30.13 ± 0.69 | 32.84 ± 0.44 | 32.25 ± 0.80 | 30.58 ± 0.53 | 30.48 ± 0.78 | 26.04 ± 1.05 | 32.14 ± 0.93 |