Table 3. Ranking of candidate reference genes in order of expression stability calculated by BestKeeper for different tissues.

Data shown as Pearson correlation coefficient (r) and standard deviation (SD).

Rank	All tissues		Gill		Hemocyte		Intestine		Leiblein		Liver		Kidney		Mantle		Gonad
	SD	r	SD	r	SD	r	SD	r	SD	r	SD	r	SD	r	SD	r	SD	r
1	RL28	EF-1α	RS25	EF-1α	TUBB	RL5	HH3	COX1	TUBB	EF-1α	RL28	PPIA	PPIA	EF-1α	RS25	EF-1α	NDUFA7	EF-1α
2	EF-1α	RS8	COX1	RS8	RS25	RL28	GAPDH	TUBB	RS8	RL28	GAPDH	RL5	GAPDH	RL5	COX1	RL5	EF-1α	RS8
3	RS25	RL28	NDUFA7	RL28	RL28	GAPDH	NDUFA7	RL5	COX1	UBE2	RL5	NDUFA7	NDUFA7	UBE2	ACT	COX1	RL5	RL28
4	RL5	RL5	EF-1α	RL5	PPIA	EF-1α	RL28	EF-1α	ACT	RL5	EF-1α	EF-1α	RL5	PPIA	RL28	PPIA	ACT	RL5
5	NDUFA7	COX1	PPIA	COX1	RS8	ACT	PPIA	RL28	UBE2	GAPDH	HH3	COX1	RL28	NDUFA7	RL5	RL28	COX1	COX1
6	PPIA	UBE2	ACT	PPIA	EF-1α	NDUFA7	COX1	PPIA	RL28	TUBB	UBE2	RS8	EF-1α	ACT	EF-1α	UBE2	RL28	PPIA
7	HH3	PPIA	HH3	ACT	ACT	RS8	ACT	RS25	RS25	RS8	RS8	RL28	ACT	RL28	RS8	NDUFA7	RS25	ACT
8	RS8	RS25	RL28	HH3	GAPDH	COX1	EF-1α	UBE2	NDUFA7	NDUFA7	ACT	RS25	HH3	RS25	GAPDH	RS8	HH3	HH3
9	GAPDH	NDUFA7	RL5	UBE2	HH3	PPIA	RS25	ACT	GAPDH	RS25	NDUFA7	ACT	TUBB	COX1	PPIA	HH3	GAPDH	UBE2
10	COX1	GAPDH	GAPDH	NDUFA7	RL5	HH3	TUBB	GAPDH	EF-1α	ACT	COX1	TUBB	RS25	GAPDH	TUBB	GAPDH	RS8	NDUFA7
11	TUBB	ACT	UBE2	RS25	NDUFA7	UBE2	RL5	RS8	HH3	COX1	RS25	UBE2	COX1	RS8	NDUFA7	RS25	UBE2	RS25
12	ACT	HH3	TUBB	GAPDH	UBE2	RS25	UBE2	NDUFA7	RL5	PPIA	TUBB	GAPDH	RS8	TUBB	UBE2	TUBB	PPIA	GAPDH
13	UBE2	TUBB	RS8	TUBB	COX1	TUBB	RS8	HH3	PPIA	HH3	PPIA	HH3	UBE2	HH3	HH3	ACT	TUBB	TUBB